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human cdna  (TaKaRa)


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    Structured Review

    TaKaRa human cdna
    Human Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+marathon+ready+cdna/Human+Lung+Marathon+-Ready+cDNA/us12600789-663-12-17
    Average 94 stars, based on 122 article reviews
    human cdna - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: Anti-erbB3 antibody
    Article Snippet: Human erbB3-Fc Protein Expression Vector A cDNA fragment of Fc fusion protein wherein the extracellular domain (SEQ ID NO:3) of human erbB3 is fused with human IgG1-Fc region (hereinbelow, described as “erbB3-Fc”) was prepared in the following manner. .. A DNA fragment encoding the amino acid sequence in the extracellular domain of erbB3 was amplified using a primer of SEQ ID NO: 7 and a primer of SEQ ID NO:8, Human lung Marathon Ready cDNA (manufactured by Clontech) as a template, and KOD Plus® DNA polymerase (manufactured by TOYOBO Co., Ltd.), by PCR performed for 35 cycles each consisting of 94° C. for 15 seconds, 60° C. for 30 seconds, and 68° C. for 2 minutes. .. This erbB3 gene fragment was digested with restriction enzymes KpnI and XbaI and inserted into an appropriate site of INPEP4 vector (manufactured by Biogen-IDEC) comprising the Fc region of human IgG, thereby preparing erbB3-Fc expression vector.

    Amplification:

    Article Title: Anti-erbB3 antibody
    Article Snippet: Human erbB3-Fc Protein Expression Vector A cDNA fragment of Fc fusion protein wherein the extracellular domain (SEQ ID NO:3) of human erbB3 is fused with human IgG1-Fc region (hereinbelow, described as “erbB3-Fc”) was prepared in the following manner. .. A DNA fragment encoding the amino acid sequence in the extracellular domain of erbB3 was amplified using a primer of SEQ ID NO: 7 and a primer of SEQ ID NO:8, Human lung Marathon Ready cDNA (manufactured by Clontech) as a template, and KOD Plus® DNA polymerase (manufactured by TOYOBO Co., Ltd.), by PCR performed for 35 cycles each consisting of 94° C. for 15 seconds, 60° C. for 30 seconds, and 68° C. for 2 minutes. .. This erbB3 gene fragment was digested with restriction enzymes KpnI and XbaI and inserted into an appropriate site of INPEP4 vector (manufactured by Biogen-IDEC) comprising the Fc region of human IgG, thereby preparing erbB3-Fc expression vector.

    Article Title: Isolation of a novel human lung-specific gene,LUNX, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer
    Article Snippet: Kyoko IWAO, Takashi WATANABE, Yoshiyuki FUJIWARA*, Koji TAKAMI, Ken KODAMA, Masahiko HIGASHIYAMA, Hideki YOKOUCHI, Kouichi OZAKI, Morito MONDEN and Akira TANIGAMI Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, Osaka, Japan Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Tokushima, Japan Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan

    Article Title: Enhanced Replication of Human T-Cell Leukemia Virus Type 1 in T Cells from Transgenic Rats Expressing Human CRM1 That Is Regulated in a Natural Manner
    Article Snippet: RT-PCR was performed by incubation for 2 min at 50°C, 30 min at 60°C, and 10 min at 95°C, followed by 50 cycles of 20 s at 95°C and 1 min at 62°C in an Applied Biosystems Prism 7700 sequence detector thermocycler with Sequence Detector software (Applied Biosystems). .. To make standard curves, the region from bp −943 to +38 of the CRM1 cDNA was amplified by PCR using Human Lung Marathon-Ready cDNA (Clontech) with adaptor primer 1 and 5′-GCTGCATGGTCTGCTAACATT-3′ and by nested PCR with adaptor primer 2 and 5′-CTGCATGGTCTGCTAACATTG-3′. .. The PCR product was cloned into the pCR 2.1 vector (Invitrogen), and a 981-base single-stranded RNA was synthesized in vitro with MegaScript T7 (Ambion).

    Article Title: Mechanisms underlying the induction of the putative human tumor suppressor GPRC5A by retinoic acid.
    Article Snippet: .. Primers were extended with superScript RT Reverse Transcriptase (GIBCO) and a major band was identified by agrose gel electrophoresis (lane 4). (B) Human lung marathon-ready cDNA (from Clonetech) was used as template for amplification of 5'-sequence of human GPRC5A with primer hEX and AP1 in marathon-ready cDNA’s adaptor and PCR products were subjected to electrophoresis. ..

    Article Title: Enhanced Replication of Human T-Cell Leukemia Virus Type 1 in T Cells from Transgenic Rats Expressing Human CRM1 That Is Regulated in a Natural Manner
    Article Snippet: RT-PCR was performed by incubation for 2 min at 50°C, 30 min at 60°C, and 10 min at 95°C, followed by 50 cycles of 20 s at 95°C and 1 min at 62°C in an Applied Biosystems Prism 7700 sequence detector thermocycler with Sequence Detector software (Applied Biosystems). .. To make standard curves, the region from bp 943 to 38 of the CRM1 cDNA was amplified by PCR using Human Lung Marathon-Ready cDNA (Clontech) with adaptor primer 1 and 5 -GCTGCATGGTCTGCTAAC ATT-3 and by nested PCR with adaptor primer 2 and 5 -CTGCATGGTCTG CTAACATTG-3 . .. The PCR product was cloned into the pCR 2.1 vector (Invitrogen), and a 981-base single-stranded RNA was synthesized in vitro with MegaScript T7 (Ambion).

    Article Title: Anti-erbB3 antibody
    Article Snippet: .. A cDNA fragment of the extracellular domain of human erbB3 was amplified using a primer of SEQ ID NO:9, a primer of SEQ ID NO:10, and Human lung Marathon Ready cDNA (manufactured by Clontech) as a template, by PCR performed for 35 cycles consisting of 94° C. for 15 seconds, 60° C. for 15 seconds, and 68° C. for 2 minutes. .. This gene fragment was digested with restriction enzymes KpnI and BglII and inserted into an appropriate site of INPEP4 vector (manufactured by Biogen-IDEC) comprising GST, thereby preparing a herbB3-GST expression vector.

    Polymerase Chain Reaction:

    Article Title: Anti-erbB3 antibody
    Article Snippet: Human erbB3-Fc Protein Expression Vector A cDNA fragment of Fc fusion protein wherein the extracellular domain (SEQ ID NO:3) of human erbB3 is fused with human IgG1-Fc region (hereinbelow, described as “erbB3-Fc”) was prepared in the following manner. .. A DNA fragment encoding the amino acid sequence in the extracellular domain of erbB3 was amplified using a primer of SEQ ID NO: 7 and a primer of SEQ ID NO:8, Human lung Marathon Ready cDNA (manufactured by Clontech) as a template, and KOD Plus® DNA polymerase (manufactured by TOYOBO Co., Ltd.), by PCR performed for 35 cycles each consisting of 94° C. for 15 seconds, 60° C. for 30 seconds, and 68° C. for 2 minutes. .. This erbB3 gene fragment was digested with restriction enzymes KpnI and XbaI and inserted into an appropriate site of INPEP4 vector (manufactured by Biogen-IDEC) comprising the Fc region of human IgG, thereby preparing erbB3-Fc expression vector.

    Article Title: Enhanced Replication of Human T-Cell Leukemia Virus Type 1 in T Cells from Transgenic Rats Expressing Human CRM1 That Is Regulated in a Natural Manner
    Article Snippet: RT-PCR was performed by incubation for 2 min at 50°C, 30 min at 60°C, and 10 min at 95°C, followed by 50 cycles of 20 s at 95°C and 1 min at 62°C in an Applied Biosystems Prism 7700 sequence detector thermocycler with Sequence Detector software (Applied Biosystems). .. To make standard curves, the region from bp −943 to +38 of the CRM1 cDNA was amplified by PCR using Human Lung Marathon-Ready cDNA (Clontech) with adaptor primer 1 and 5′-GCTGCATGGTCTGCTAACATT-3′ and by nested PCR with adaptor primer 2 and 5′-CTGCATGGTCTGCTAACATTG-3′. .. The PCR product was cloned into the pCR 2.1 vector (Invitrogen), and a 981-base single-stranded RNA was synthesized in vitro with MegaScript T7 (Ambion).

    Article Title: Mechanisms underlying the induction of the putative human tumor suppressor GPRC5A by retinoic acid.
    Article Snippet: .. Primers were extended with superScript RT Reverse Transcriptase (GIBCO) and a major band was identified by agrose gel electrophoresis (lane 4). (B) Human lung marathon-ready cDNA (from Clonetech) was used as template for amplification of 5'-sequence of human GPRC5A with primer hEX and AP1 in marathon-ready cDNA’s adaptor and PCR products were subjected to electrophoresis. ..

    Article Title: Enhanced Replication of Human T-Cell Leukemia Virus Type 1 in T Cells from Transgenic Rats Expressing Human CRM1 That Is Regulated in a Natural Manner
    Article Snippet: RT-PCR was performed by incubation for 2 min at 50°C, 30 min at 60°C, and 10 min at 95°C, followed by 50 cycles of 20 s at 95°C and 1 min at 62°C in an Applied Biosystems Prism 7700 sequence detector thermocycler with Sequence Detector software (Applied Biosystems). .. To make standard curves, the region from bp 943 to 38 of the CRM1 cDNA was amplified by PCR using Human Lung Marathon-Ready cDNA (Clontech) with adaptor primer 1 and 5 -GCTGCATGGTCTGCTAAC ATT-3 and by nested PCR with adaptor primer 2 and 5 -CTGCATGGTCTG CTAACATTG-3 . .. The PCR product was cloned into the pCR 2.1 vector (Invitrogen), and a 981-base single-stranded RNA was synthesized in vitro with MegaScript T7 (Ambion).

    Article Title: Anti-erbB3 antibody
    Article Snippet: .. A cDNA fragment of the extracellular domain of human erbB3 was amplified using a primer of SEQ ID NO:9, a primer of SEQ ID NO:10, and Human lung Marathon Ready cDNA (manufactured by Clontech) as a template, by PCR performed for 35 cycles consisting of 94° C. for 15 seconds, 60° C. for 15 seconds, and 68° C. for 2 minutes. .. This gene fragment was digested with restriction enzymes KpnI and BglII and inserted into an appropriate site of INPEP4 vector (manufactured by Biogen-IDEC) comprising GST, thereby preparing a herbB3-GST expression vector.

    Nested PCR:

    Article Title: Enhanced Replication of Human T-Cell Leukemia Virus Type 1 in T Cells from Transgenic Rats Expressing Human CRM1 That Is Regulated in a Natural Manner
    Article Snippet: RT-PCR was performed by incubation for 2 min at 50°C, 30 min at 60°C, and 10 min at 95°C, followed by 50 cycles of 20 s at 95°C and 1 min at 62°C in an Applied Biosystems Prism 7700 sequence detector thermocycler with Sequence Detector software (Applied Biosystems). .. To make standard curves, the region from bp −943 to +38 of the CRM1 cDNA was amplified by PCR using Human Lung Marathon-Ready cDNA (Clontech) with adaptor primer 1 and 5′-GCTGCATGGTCTGCTAACATT-3′ and by nested PCR with adaptor primer 2 and 5′-CTGCATGGTCTGCTAACATTG-3′. .. The PCR product was cloned into the pCR 2.1 vector (Invitrogen), and a 981-base single-stranded RNA was synthesized in vitro with MegaScript T7 (Ambion).

    Article Title: Enhanced Replication of Human T-Cell Leukemia Virus Type 1 in T Cells from Transgenic Rats Expressing Human CRM1 That Is Regulated in a Natural Manner
    Article Snippet: RT-PCR was performed by incubation for 2 min at 50°C, 30 min at 60°C, and 10 min at 95°C, followed by 50 cycles of 20 s at 95°C and 1 min at 62°C in an Applied Biosystems Prism 7700 sequence detector thermocycler with Sequence Detector software (Applied Biosystems). .. To make standard curves, the region from bp 943 to 38 of the CRM1 cDNA was amplified by PCR using Human Lung Marathon-Ready cDNA (Clontech) with adaptor primer 1 and 5 -GCTGCATGGTCTGCTAAC ATT-3 and by nested PCR with adaptor primer 2 and 5 -CTGCATGGTCTG CTAACATTG-3 . .. The PCR product was cloned into the pCR 2.1 vector (Invitrogen), and a 981-base single-stranded RNA was synthesized in vitro with MegaScript T7 (Ambion).

    Reverse Transcription:

    Article Title: Mechanisms underlying the induction of the putative human tumor suppressor GPRC5A by retinoic acid.
    Article Snippet: .. Primers were extended with superScript RT Reverse Transcriptase (GIBCO) and a major band was identified by agrose gel electrophoresis (lane 4). (B) Human lung marathon-ready cDNA (from Clonetech) was used as template for amplification of 5'-sequence of human GPRC5A with primer hEX and AP1 in marathon-ready cDNA’s adaptor and PCR products were subjected to electrophoresis. ..

    Nucleic Acid Electrophoresis:

    Article Title: Mechanisms underlying the induction of the putative human tumor suppressor GPRC5A by retinoic acid.
    Article Snippet: .. Primers were extended with superScript RT Reverse Transcriptase (GIBCO) and a major band was identified by agrose gel electrophoresis (lane 4). (B) Human lung marathon-ready cDNA (from Clonetech) was used as template for amplification of 5'-sequence of human GPRC5A with primer hEX and AP1 in marathon-ready cDNA’s adaptor and PCR products were subjected to electrophoresis. ..

    Electrophoresis:

    Article Title: Mechanisms underlying the induction of the putative human tumor suppressor GPRC5A by retinoic acid.
    Article Snippet: .. Primers were extended with superScript RT Reverse Transcriptase (GIBCO) and a major band was identified by agrose gel electrophoresis (lane 4). (B) Human lung marathon-ready cDNA (from Clonetech) was used as template for amplification of 5'-sequence of human GPRC5A with primer hEX and AP1 in marathon-ready cDNA’s adaptor and PCR products were subjected to electrophoresis. ..



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    Image Search Results


    Journal: Molecules

    Article Title: Biological Activity of Pseudovitamin B 12 on Cobalamin-Dependent Methylmalonyl-CoA Mutase and Methionine Synthase in Mammalian Cultured COS-7 Cells

    doi: 10.3390/molecules25143268

    Figure Lengend Snippet: Total and holo-MS activity in MS cDNA-transfected cells grown with or without OH-Cbl and (Ade)OH-Cba.

    Article Snippet: First, the cDNA fragments encoding human MS (hMS) and human TCII (hTCII) containing UTR were amplified from Human Marathon-Ready cDNA (brain, cerebral cortex; Clontech, CA, USA) by PCR using the following primer sets: hMS-F1 (5′-AGCCAACGGGAGGCGTCAAAAGACC-3′) and hMS-R1 (5′-CAGGAAGACCCTGCTCCTCTACAAGG-3′), and hTCII-F1 (5′-GGAGTCTTTCCCGATTCTTGCT-3′) and hTCII-R1 (5′-ACCACAGAACGAGTGGTCTTCA-3′).

    Techniques: Activity Assay

    Western blotting of MS protein in MS cDNA-transfected cells grown with or without OH-Cbl and (Ade)OH-Cba. Cells were cultured in the medium with or without 1 µM OH-Cbl or 1 µM (Ade)OH-Cba for 2 days. Other procedures were done as shown in . Immunoreactive bands with higher and lower molecular weights are derived from exogenous and endogenous MS proteins, respectively. Data are typical immunoreactive patterns of the MS protein from three independent Western blot analyses of treated cells.

    Journal: Molecules

    Article Title: Biological Activity of Pseudovitamin B 12 on Cobalamin-Dependent Methylmalonyl-CoA Mutase and Methionine Synthase in Mammalian Cultured COS-7 Cells

    doi: 10.3390/molecules25143268

    Figure Lengend Snippet: Western blotting of MS protein in MS cDNA-transfected cells grown with or without OH-Cbl and (Ade)OH-Cba. Cells were cultured in the medium with or without 1 µM OH-Cbl or 1 µM (Ade)OH-Cba for 2 days. Other procedures were done as shown in . Immunoreactive bands with higher and lower molecular weights are derived from exogenous and endogenous MS proteins, respectively. Data are typical immunoreactive patterns of the MS protein from three independent Western blot analyses of treated cells.

    Article Snippet: First, the cDNA fragments encoding human MS (hMS) and human TCII (hTCII) containing UTR were amplified from Human Marathon-Ready cDNA (brain, cerebral cortex; Clontech, CA, USA) by PCR using the following primer sets: hMS-F1 (5′-AGCCAACGGGAGGCGTCAAAAGACC-3′) and hMS-R1 (5′-CAGGAAGACCCTGCTCCTCTACAAGG-3′), and hTCII-F1 (5′-GGAGTCTTTCCCGATTCTTGCT-3′) and hTCII-R1 (5′-ACCACAGAACGAGTGGTCTTCA-3′).

    Techniques: Western Blot, Transfection, Cell Culture, Derivative Assay

    Effects of various concentrations of OH-Cbl ( A ) or (Ade)OH-Cba ( B ) on MS activity in MS cDNA-transfected cells. A cell homogenate of MS cDNA-transfected cells grown without OH-Cbl was used as an apo-MS preparation. MS activity was assayed in the reaction mixture containing various concentrations of OH-Cbl (0.1, 0.25, 0.5, 1, 2.5, 5, and 10 μM) or (Ade)OH-Cba (2, 5, 10, 25, and 50 μM).

    Journal: Molecules

    Article Title: Biological Activity of Pseudovitamin B 12 on Cobalamin-Dependent Methylmalonyl-CoA Mutase and Methionine Synthase in Mammalian Cultured COS-7 Cells

    doi: 10.3390/molecules25143268

    Figure Lengend Snippet: Effects of various concentrations of OH-Cbl ( A ) or (Ade)OH-Cba ( B ) on MS activity in MS cDNA-transfected cells. A cell homogenate of MS cDNA-transfected cells grown without OH-Cbl was used as an apo-MS preparation. MS activity was assayed in the reaction mixture containing various concentrations of OH-Cbl (0.1, 0.25, 0.5, 1, 2.5, 5, and 10 μM) or (Ade)OH-Cba (2, 5, 10, 25, and 50 μM).

    Article Snippet: First, the cDNA fragments encoding human MS (hMS) and human TCII (hTCII) containing UTR were amplified from Human Marathon-Ready cDNA (brain, cerebral cortex; Clontech, CA, USA) by PCR using the following primer sets: hMS-F1 (5′-AGCCAACGGGAGGCGTCAAAAGACC-3′) and hMS-R1 (5′-CAGGAAGACCCTGCTCCTCTACAAGG-3′), and hTCII-F1 (5′-GGAGTCTTTCCCGATTCTTGCT-3′) and hTCII-R1 (5′-ACCACAGAACGAGTGGTCTTCA-3′).

    Techniques: Activity Assay, Transfection